Annals of Maxillofacial Surgery

ORIGINAL ARTICLE - COMPARATIVE STUDY
Year
: 2021  |  Volume : 11  |  Issue : 1  |  Page : 6--11

Prevalence of Human Papillomavirus (HPV) 16 and 18 in oral malignant and potentially malignant disorders: A polymerase chain reaction analysis – A comparative study


Sathya Sri1, Pratibha Ramani2, Priya Premkumar1, Vijayalakshmi Ramshankar3, Abilasha Ramasubramanian2, Reshma Poothakulath Krishnan2 
1 Department of Oral Pathology, CSI Dental College, Madurai, Tamil Nadu, India
2 Department of Oral Pathology, Saveetha Dental College and Hospitals, Chennai, Tamil Nadu, India
3 Department of Preventive Oncology (Molecular Research Division), Cancer Institute (WIA), Chennai, Tamil Nadu, India

Correspondence Address:
Dr. Pratibha Ramani
Saveetha Dental College and Hospitals, Chennai - 600 077, Tamil Nadu
India

Introduction: Human papillomavirus (HPV) are now being increasingly associated as a cause of oral squamous cell carcinomas (OSCC). This study was designed to evaluate the prevalence of HPV in Pelizaeus-Merzbacher disease (PMD) and OSCC using polymerase chain reaction that might help in better understanding of the role played by this virus in the oncogenic process even from its evolution stage. Materials and Methods: Formalin-fixed paraffin-embedded tissue samples (n = 40) of OSCC and mild, moderate, and severe dysplasia were used for this study. DNA was quantified and checked for purity spectrophotometrically. Statistical analysis was performed using SPSS software and statistical significance was assessed using Fischer's exact test (p < 0.05 was considered significant). Results: High-risk (HR)-HPV-16 was found to be positive in 35% of OSCC cases which showed a statistically significant association of HPV 16 with OSCC. Verrucous carcinoma had predominant HPV 16 infection (60%), followed by SCC with 40%. However, this association was not statistically significant. None of the OSCC samples were infected with HPV 18. Among the PMD, we found only 5% showing HR-HPV 16 infection which was not significant. Discussion: Although OSCC is attributed to tobacco and alcohol consumption, a significant proportion of OSCC cases have been demonstrated to contain HPV types. The high-risk HPV type 16 tends to be the most predominant type detected in cases of OSCC.


How to cite this article:
Sri S, Ramani P, Premkumar P, Ramshankar V, Ramasubramanian A, Krishnan RP. Prevalence of Human Papillomavirus (HPV) 16 and 18 in oral malignant and potentially malignant disorders: A polymerase chain reaction analysis – A comparative study.Ann Maxillofac Surg 2021;11:6-11


How to cite this URL:
Sri S, Ramani P, Premkumar P, Ramshankar V, Ramasubramanian A, Krishnan RP. Prevalence of Human Papillomavirus (HPV) 16 and 18 in oral malignant and potentially malignant disorders: A polymerase chain reaction analysis – A comparative study. Ann Maxillofac Surg [serial online] 2021 [cited 2021 Nov 28 ];11:6-11
Available from: https://www.amsjournal.com/article.asp?issn=2231-0746;year=2021;volume=11;issue=1;spage=6;epage=11;aulast=Sri;type=0